Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination.
Identifieur interne : 000074 ( Main/Exploration ); précédent : 000073; suivant : 000075Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination.
Auteurs : Huanhuan Li [République populaire de Chine] ; Zhenjie Dong [République populaire de Chine] ; Chao Ma [République populaire de Chine] ; Xiubin Tian [République populaire de Chine] ; Zengjun Qi [République populaire de Chine] ; Nan Wu [République populaire de Chine] ; Bernd Friebe [États-Unis] ; Zhiguo Xiang [République populaire de Chine] ; Qing Xia [République populaire de Chine] ; Wenxuan Liu [République populaire de Chine] ; Tianya Li [République populaire de Chine]Source :
- International journal of molecular sciences [ 1422-0067 ] ; 2019.
Descripteurs français
- KwdFr :
- Basidiomycota (MeSH), Cartographie chromosomique (MeSH), Gènes de plante (MeSH), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Marqueurs génétiques (MeSH), Phénotype (MeSH), Points de cassure de chromosome (MeSH), Recombinaison génétique (MeSH), Résistance à la maladie (génétique), Triticum (génétique), Triticum (microbiologie).
- MESH :
English descriptors
- KwdEn :
- Basidiomycota (MeSH), Chromosome Breakpoints (MeSH), Chromosome Mapping (MeSH), Disease Resistance (genetics), Genes, Plant (MeSH), Genetic Markers (MeSH), Phenotype (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Recombination, Genetic (MeSH), Triticum (genetics), Triticum (microbiology).
- MESH :
- chemical : Genetic Markers.
- genetics : Disease Resistance, Plant Diseases, Triticum.
- microbiology : Plant Diseases, Triticum.
- Basidiomycota, Chromosome Breakpoints, Chromosome Mapping, Genes, Plant, Phenotype, Recombination, Genetic.
Abstract
Wheat stem rust caused by Puccinia graminis f. sp. tritici (Pgt) had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. Sr52, derived from Dasypyrum villosum and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-D. villosum Robertsonian translocation T6AS·6V#3L, the only stock carrying Sr52 released to experimental and breeding programs so far, was crossed with a CS ph1b mutant to induce recombinants with shortened 6V#3L chromosome segments locating Sr52. Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using in situ hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene Sr52 was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the Sr52-located interval of 6V#3L were confirmed to be diagnostic markers for selection of Sr52 introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding.
DOI: 10.3390/ijms20194887
PubMed: 31581639
PubMed Central: PMC6801782
Affiliations:
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wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. 2017201043@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Friebe, Bernd" sort="Friebe, Bernd" uniqKey="Friebe B" first="Bernd" last="Friebe">Bernd Friebe</name><affiliation wicri:level="2"><nlm:affiliation>Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA. friebe@ksu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Xiang, Zhiguo" sort="Xiang, Zhiguo" uniqKey="Xiang Z" first="Zhiguo" last="Xiang">Zhiguo Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiangwheat@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Henan Academy of Agricultural Sciences, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Qing" sort="Xia, Qing" uniqKey="Xia Q" first="Qing" last="Xia">Qing Xia</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. qingxia412@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Wenxuan" sort="Liu, Wenxuan" uniqKey="Liu W" first="Wenxuan" last="Liu">Wenxuan Liu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. liuwenxuan@henau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Li, Tianya" sort="Li, Tianya" uniqKey="Li T" first="Tianya" last="Li">Tianya Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110000, China. litianya11@syau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Shenyang Agricultural University, Shenyang 110000</wicri:regionArea><wicri:noRegion>Shenyang 110000</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31581639</idno><idno type="pmid">31581639</idno><idno type="doi">10.3390/ijms20194887</idno><idno type="pmc">PMC6801782</idno><idno type="wicri:Area/Main/Corpus">000071</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000071</idno><idno type="wicri:Area/Main/Curation">000071</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000071</idno><idno type="wicri:Area/Main/Exploration">000071</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Physical Mapping of Stem Rust Resistance Gene Sr52 from <i>Dasypyrum villosum</i> Based on <i>ph1b</i>-Induced Homoeologous Recombination.</title><author><name sortKey="Li, Huanhuan" sort="Li, Huanhuan" uniqKey="Li H" first="Huanhuan" last="Li">Huanhuan Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. lihuanhuanhappy@henau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Zhenjie" sort="Dong, Zhenjie" uniqKey="Dong Z" first="Zhenjie" last="Dong">Zhenjie Dong</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. zhenjiedong@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Chao" sort="Ma, Chao" uniqKey="Ma C" first="Chao" last="Ma">Chao Ma</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. machao0813@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Xiubin" sort="Tian, Xiubin" uniqKey="Tian X" first="Xiubin" last="Tian">Xiubin Tian</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. XiubinTian2019@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Qi, Zengjun" sort="Qi, Zengjun" uniqKey="Qi Z" first="Zengjun" last="Qi">Zengjun Qi</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. zjqi@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Nan" sort="Wu, Nan" uniqKey="Wu N" first="Nan" last="Wu">Nan Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. 2017201043@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Friebe, Bernd" sort="Friebe, Bernd" uniqKey="Friebe B" first="Bernd" last="Friebe">Bernd Friebe</name><affiliation wicri:level="2"><nlm:affiliation>Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA. friebe@ksu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Xiang, Zhiguo" sort="Xiang, Zhiguo" uniqKey="Xiang Z" first="Zhiguo" last="Xiang">Zhiguo Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiangwheat@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Henan Academy of Agricultural Sciences, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Qing" sort="Xia, Qing" uniqKey="Xia Q" first="Qing" last="Xia">Qing Xia</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. qingxia412@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Wenxuan" sort="Liu, Wenxuan" uniqKey="Liu W" first="Wenxuan" last="Liu">Wenxuan Liu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. liuwenxuan@henau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002</wicri:regionArea><wicri:noRegion>Zhengzhou 450002</wicri:noRegion></affiliation></author><author><name sortKey="Li, Tianya" sort="Li, Tianya" uniqKey="Li T" first="Tianya" last="Li">Tianya Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110000, China. litianya11@syau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Shenyang Agricultural University, Shenyang 110000</wicri:regionArea><wicri:noRegion>Shenyang 110000</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of molecular sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (MeSH)</term><term>Chromosome Breakpoints (MeSH)</term><term>Chromosome Mapping (MeSH)</term><term>Disease Resistance (genetics)</term><term>Genes, Plant (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Recombination, Genetic (MeSH)</term><term>Triticum (genetics)</term><term>Triticum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (MeSH)</term><term>Cartographie chromosomique (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Marqueurs génétiques (MeSH)</term><term>Phénotype (MeSH)</term><term>Points de cassure de chromosome (MeSH)</term><term>Recombinaison génétique (MeSH)</term><term>Résistance à la maladie (génétique)</term><term>Triticum (génétique)</term><term>Triticum (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term><term>Résistance à la maladie</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Basidiomycota</term><term>Chromosome Breakpoints</term><term>Chromosome Mapping</term><term>Genes, Plant</term><term>Phenotype</term><term>Recombination, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basidiomycota</term><term>Cartographie chromosomique</term><term>Gènes de plante</term><term>Marqueurs génétiques</term><term>Phénotype</term><term>Points de cassure de chromosome</term><term>Recombinaison génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wheat stem rust caused by <i>Puccinia graminis</i> f. sp. <i>tritici</i> (<i>Pgt</i>) had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. <i>Sr52</i>, derived from <i>Dasypyrum villosum</i> and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-<i>D</i>. <i>villosum</i> Robertsonian translocation T6AS·6V#3L, the only stock carrying <i>Sr52</i> released to experimental and breeding programs so far, was crossed with a CS <i>ph1b</i> mutant to induce recombinants with shortened 6V#3L chromosome segments locating <i>Sr52</i>. Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using <i>in situ</i> hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene <i>Sr52</i> was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the <i>Sr52</i>-located interval of 6V#3L were confirmed to be diagnostic markers for selection of <i>Sr52</i> introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31581639</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>12</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>19</Issue><PubDate><Year>2019</Year><Month>Oct</Month><Day>02</Day></PubDate></JournalIssue><Title>International journal of molecular sciences</Title><ISOAbbreviation>Int J Mol Sci</ISOAbbreviation></Journal><ArticleTitle>Physical Mapping of Stem Rust Resistance Gene Sr52 from <i>Dasypyrum villosum</i> Based on <i>ph1b</i>-Induced Homoeologous Recombination.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E4887</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms20194887</ELocationID><Abstract><AbstractText>Wheat stem rust caused by <i>Puccinia graminis</i> f. sp. <i>tritici</i> (<i>Pgt</i>) had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. <i>Sr52</i>, derived from <i>Dasypyrum villosum</i> and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-<i>D</i>. <i>villosum</i> Robertsonian translocation T6AS·6V#3L, the only stock carrying <i>Sr52</i> released to experimental and breeding programs so far, was crossed with a CS <i>ph1b</i> mutant to induce recombinants with shortened 6V#3L chromosome segments locating <i>Sr52</i>. Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using <i>in situ</i> hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene <i>Sr52</i> was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the <i>Sr52</i>-located interval of 6V#3L were confirmed to be diagnostic markers for selection of <i>Sr52</i> introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Huanhuan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. lihuanhuanhappy@henau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Zhenjie</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. zhenjiedong@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. machao0813@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Xiubin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. XiubinTian2019@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Zengjun</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. zjqi@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Nan</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. 2017201043@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Friebe</LastName><ForeName>Bernd</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA. friebe@ksu.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Zhiguo</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiangwheat@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Qing</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. qingxia412@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Wenxuan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. liuwenxuan@henau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Tianya</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Shenyang Agricultural University, Shenyang 110000, China. litianya11@syau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>No. 31801361 and No. 31571658</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>No. 192102110011</GrantID><Agency>Scientific and Technological Research Project of Henan Province of China</Agency><Country></Country></Grant><Grant><GrantID>No. 161100110400</GrantID><Agency>Henan Province Special Major Science and Technology Project</Agency><Country></Country></Grant><Grant><GrantID>No. 39990022</GrantID><Agency>State Key Laboratory of Wheat and Maize Crop Science at Henan Agricultural University, China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Mol Sci</MedlineTA><NlmUniqueID>101092791</NlmUniqueID><ISSNLinking>1422-0067</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="Y">Basidiomycota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056905" MajorTopicYN="N">Chromosome Breakpoints</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="Y">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011995" MajorTopicYN="Y">Recombination, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">D. villosum</Keyword><Keyword MajorTopicYN="N">Sr52</Keyword><Keyword MajorTopicYN="N">common wheat</Keyword><Keyword MajorTopicYN="N">molecular marker</Keyword><Keyword MajorTopicYN="N">small segment translocation line</Keyword><Keyword MajorTopicYN="N">stem rust resistance</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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last="Dong">Zhenjie Dong</name><name sortKey="Li, Tianya" sort="Li, Tianya" uniqKey="Li T" first="Tianya" last="Li">Tianya Li</name><name sortKey="Liu, Wenxuan" sort="Liu, Wenxuan" uniqKey="Liu W" first="Wenxuan" last="Liu">Wenxuan Liu</name><name sortKey="Ma, Chao" sort="Ma, Chao" uniqKey="Ma C" first="Chao" last="Ma">Chao Ma</name><name sortKey="Qi, Zengjun" sort="Qi, Zengjun" uniqKey="Qi Z" first="Zengjun" last="Qi">Zengjun Qi</name><name sortKey="Tian, Xiubin" sort="Tian, Xiubin" uniqKey="Tian X" first="Xiubin" last="Tian">Xiubin Tian</name><name sortKey="Wu, Nan" sort="Wu, Nan" uniqKey="Wu N" first="Nan" last="Wu">Nan Wu</name><name sortKey="Xia, Qing" sort="Xia, Qing" uniqKey="Xia Q" first="Qing" last="Xia">Qing Xia</name><name sortKey="Xiang, Zhiguo" sort="Xiang, Zhiguo" uniqKey="Xiang Z" first="Zhiguo" last="Xiang">Zhiguo Xiang</name></country><country name="États-Unis"><region name="Kansas"><name sortKey="Friebe, Bernd" sort="Friebe, Bernd" uniqKey="Friebe B" first="Bernd" 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